Human S1PR5 Sphingosine 1-phosphate receptor 5 ELISA Kit

Overview Quick Details

Place of Origin:
Brand Name:
Model Number:
Blood Testing Equipments
Instrument classification:
Class I
Product Spec:
For research
Detection Range:
Natural and recombinant human Sphingosine 1-phosphate receptor 5
Assay Type:
Research Area:
Sample Type:
Serum,plasma,tissue homogenates,supernates and biological fluids

Packaging & Delivery

Selling Units:
Single item
Single package size:
27X19X16 cm
Single gross weight:
2.0 kg
Package Type:
Ice bags in foam box, carton box outside
Picture Example:
Lead Time :
Quantity(Boxes) 1 – 10 >10 Est. Time(days) 7 To be negotiated

Online Customization

E2206Hu is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Sphingosine 1-phosphate receptor 5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Sphingosine 1-phosphate receptor 5. The ELISA analytical biochemical technique of the E2206Hu kit is based on Sphingosine 1-phosphate receptor 5 antibody-Sphingosine 1-phosphate receptor 5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sphingosine 1-phosphate receptor 5 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Sphingosine 1-phosphate receptor 5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).





Assay plate


Stored at -20℃





Sample Diluent

1 × 20ml


Assay Diluent A

1 × 10ml


Assay Diluent B

1 × 10ml


Detection Reagent A

1 × 120μl


Detection Reagent B

1 × 120μl


Wash Buffer(25 x concentrate)

1 × 30ml

Stored at 4℃



1 × 10ml


Stop Solution

1 × 10ml


Plate sealer for 96 wells



Storageof the kits

TheAssay Plate, Standard, Detection Reagent A and Detection Reagent Bshould be stored at -20°Cupon being received. After receiving the kit,Substrateshould be always stored at4°C.Other reagents are kept according to the labels on vials.But for long term storage, please keep the whole kit at -20°C(Except the substrate). The unused strips should be kept in a sealed bagwith the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.


Sample collection and storage

Serum- Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20°Cor -80°C.

Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g at 2°C- 8°Cwithin 30 minutes of collection. Store samples at -20°Cor -80°C. Avoid repeated freeze-thaw cycles.

Tissue homogenates- The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤-20°CAfter two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g. Remove the supernate and assay immediately or aliquot and store at ≤-20°C.

Cell culture supernates andOther biological fluids- Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°Cor -80°C. Avoid repeated freeze-thaw cycles.

All of our elisa kits packed with icebag and use foam box to keep the temperature.Carton outside.

We sent elisa kitBy TNT/DHL/UPS/FEDEX which is direct to your hands and normally it takes 3-5 days toarrive your place.

For more infomation about the delivery,please send company introduce tocrystal(hangzhou ever-power transmission co. ltd. is specialist in power transmission)sunlongbiotech.comOr say " Hi " to Whatsapp:+86 13738079864

Company Information

Our team:

We have many years experiences in producing elisa kits and sold to over 40countries ,

any technical questions or product inquiry,pleasecompany introduce to crystal(hangzhou ever-power transmission co. ltd. is specialist in power transmission)

Or say " Hi " to Whatsapp:+86 13738079864.You will have a easy communication with us!

Contact us

Contact us: crystal(hangzhou ever-power transmission co. ltd. is specialist in power transmission)

Any one who want to buy our elisa kits or want to be a distributor of us.Kindly company introduce us to crystal(hangzhou ever-power transmission co. ltd. is specialist in power transmission)sunlongbiotech.comOr say " Hi " to Whatsapp:+86 13738079864Mob:+8613738079864