Overview Quick Details
Packaging & Delivery
E0606Ra is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Melanin-concentrating hormone receptor 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Melanin-concentrating hormone receptor 1. The ELISA analytical biochemical technique of the E0606Ra kit is based on Melanin-concentrating hormone receptor 1 antibody-Melanin-concentrating hormone receptor 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Melanin-concentrating hormone receptor 1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Melanin-concentrating hormone receptor 1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Advantages of these ELISA kits:
- Thousands of kits provided
- Optimized for sensitive, accurate, and consistent performance
- Thorough instructions to complete protocol in 2.5 to 4 hours (varies by kit)
- Validated for typical sample types (e.g., serum, plasma, supernatant, lysates)
Details of the products picture
Materials provided with the kit
Stored at -20°C
1 × 20ml
Assay Diluent A
1 × 10ml
Assay Diluent B
1 × 10ml
Detection Reagent A
Detection Reagent B
1 × 120μl
Wash Buffer(25 x concentrate)
1 × 30ml
Stored at 4°C
1 × 10ml
1 × 10ml
Plate sealer for 96 wells
Most kits are effective for several sample types (e.g., serum, plasma, and cell culture supernatant) or cell lysate, tissue homogenate, urine, as appropriate to the target. Kits are available for use with human, mouse, rat, monkey, bovine, and porcine (swine) samples.
Storageof the kits
TheAssay Plate, Standard, Detection Reagent A and Detection Reagent Bshould be stored at -20°Cupon being received. After receiving the kit,Substrateshould be always stored at4°C.Other reagents are kept according to the labels on vials.But for long term storage, please keep the whole kit at -20°C(Except the substrate). The unused strips should be kept in a sealed bagwith the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Sample collection and storage
Serum- Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20°Cor -80°C.
Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g at 2°C- 8°Cwithin 30 minutes of collection. Store samples at -20°Cor -80°C. Avoid repeated freeze-thaw cycles.
Tissue homogenates- The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤-20°CAfter two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g. Remove the supernate and assay immediately or aliquot and store at ≤-20°C.
Cell culture supernates andOther biological fluids- Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°Cor -80°C. Avoid repeated freeze-thaw cycles.
All of our elisa kits packed with icebag and use foam box to keep the temperature.Carton outside.
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